hydrocyclone cell harvesting
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hydrocyclone cell harvesting

Use of Hydrocyclones for Mammalian Cell Retention ...

2006-9-6  As the overflow is commonly used for product harvest and cells are discarded, the application of the hydrocyclone has no detrimental effect on the reactor perfusion system. The results indicate that only cells passing from the primary vortex downwards into the inner secondary vortex and from there upwards could be damaged.

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Hydrocyclones as cell retention device for CHO perfusion ...

Hydrocyclones as cell retention devices for an N-1 perfusion bioreactor linked to a continuous-flow stirred tank production bioreactor

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Hydrocyclones for single-use perfusion application

2019-12-6  Cell diameter, harvest 0 0.4 0.8 1.2 0 20 40 60 80 100 0 5 10 15 20 ... Why is a hydrocyclone a suitable cell retention device for perfusion applications? Perfusion runs with the hydrocyclone coupled to a 50-L single-use bioreactor bag Characterization of the hydrocyclone Conclusion

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9.5.1.9: Hydrocyclone Separation of Targeted

2016-5-10  – Hydrocyclone: separates components in fluid mixture – density and/or size – Nanostructured adsorbents (NA): harvest lipid Program tasks – Establish baseline understanding of hydrocyclone separation of lipid products from algal growth media – Develop separation process metrics based on lipid products from algal growth media

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Use of Hydrocyclones for Mammalian Cell Retention ...

As the overflow is commonly used for product harvest and cells are discarded, the application of the hydrocyclone has no detrimental effect on the reactor perfusion system. The results indicate...

More

(PDF) Separation of CHO cells using hydrocyclones

A hydrocyclone is a macroscale separation device employed in various industries, with many advantages, including high-throughput and low operational costs. Translating these advantages to ...

More

Harvesting of microalgae by centrifugation for biodiesel ...

2020-10-1  Gregg et al. reported a low harvesting efficiency for particles smaller than 400 μm using hydrocyclones for the harvesting of green algae. In terms of cell damage, a hydrocyclone is predicted to cause a certain level of mechanical disruption, however, there is a lack of specialized studies on its effect for large colonial microalgae .

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Separation of CHO cells using hydrocyclones SpringerLink

2007-11-14  High cell separation efficiencies, varying from 97.9% to 99.9%, were obtained for 5 out of 6 hydrocyclone configurations. The geometry 3010 (D u = 3.0 mm and D o = 1.0 mm) presented a flow ratio of 100%, diverting the total feed flow to the underflow, indicating that this configuration is inappropriate to perform any kind of cell separation. . Loss of viability was within the range of 2.9–9 ...

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Data Hydrocyclone SBL2001-e - StuDocu

hydrocyclone mammalian cell retention device for and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification material

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A two-step microengineered system for high-density cell ...

2021-1-1  The inertial forces focus particles/cells close to the channel wall; (B) Schematic diagram of the integrated miniaturized hydrocyclone/spiral microchannel system. Cell culture is pumped into the hydrocyclone first for pre-sorting, and then the cell poor stream is pumped into the multiplexed spiral microchannel device for concentration.

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9.5.1.9: Hydrocyclone Separation of Targeted

2016-5-10  – Hydrocyclone: separates components in fluid mixture – density and/or size – Nanostructured adsorbents (NA): harvest lipid Program tasks – Establish baseline understanding of hydrocyclone separation of lipid products from algal growth media – Develop separation process metrics based on lipid products from algal growth media

More

(PDF) Separation of CHO cells using hydrocyclones

A hydrocyclone is a macroscale separation device employed in various industries, with many advantages, including high-throughput and low operational costs. Translating these advantages to ...

More

Harvesting of microalgae by centrifugation for biodiesel ...

2020-10-1  Gregg et al. reported a low harvesting efficiency for particles smaller than 400 μm using hydrocyclones for the harvesting of green algae. In terms of cell damage, a hydrocyclone is predicted to cause a certain level of mechanical disruption, however, there is a lack of specialized studies on its effect for large colonial microalgae .

More

Separation of CHO cells using hydrocyclones. - Abstract ...

2007-11-14  The cell number balances (comparison of the amount of cells leaving the hydrocyclone through the underflow and overflow streams with the amount of cells fed to the equipment) closed satisfactorily, with an average deviation of ±9.9%, which is approximately the same deviation associated to the cell counting technique in Neubauer chambers used ...

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Separation of CHO cells using hydrocyclones SpringerLink

2007-11-14  High cell separation efficiencies, varying from 97.9% to 99.9%, were obtained for 5 out of 6 hydrocyclone configurations. The geometry 3010 (D u = 3.0 mm and D o = 1.0 mm) presented a flow ratio of 100%, diverting the total feed flow to the underflow, indicating that this configuration is inappropriate to perform any kind of cell separation. . Loss of viability was within the range of 2.9–9 ...

More

Hydrocyclone for mAb production in a perfusion single

2020-10-20  For the first time, a hydrocyclone specially designed for mammalian cell separation is reported to enable perfusion processes at cell densities in the range of 20 to 50 million/mL for 20 to 25 days. Also for the first time, a HC is connected to a single-use bioreactor.

More

(PDF) Separation of CHO cells using hydrocyclones ...

Separation of CHO cells using hydrocyclones. Rodrigo Pinto. Related Papers. Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) ... Characterizing hydrocyclone performance for grit removal from wastewater treatment activated sludge plants.

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separation: Application to primary harvesting of ...

2017-6-2  Brief comparison has been made between the conventional techniques for primary harvesting of microalgae based on the concentration factor and power consumption in the table S3. Technique Concentration factor Power consumption (kWh/m3) Continuous Ref Macro-hydrocyclone 4 0.3 Yes (1) Decanter bowl centrifuge 11 8 No (2) Vacuum filter 2-18.5 - Yes ...

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Data Hydrocyclone SBL2001-e - StuDocu

hydrocyclone mammalian cell retention device for and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification material

More

A two-step microengineered system for high-density cell ...

2021-1-1  The inertial forces focus particles/cells close to the channel wall; (B) Schematic diagram of the integrated miniaturized hydrocyclone/spiral microchannel system. Cell culture is pumped into the hydrocyclone first for pre-sorting, and then the cell poor stream is pumped into the multiplexed spiral microchannel device for concentration.

More

9.5.1.9: Hydrocyclone Separation of Targeted

2016-5-10  – Hydrocyclone: separates components in fluid mixture – density and/or size – Nanostructured adsorbents (NA): harvest lipid Program tasks – Establish baseline understanding of hydrocyclone separation of lipid products from algal growth media – Develop separation process metrics based on lipid products from algal growth media

More

Hydrocyclone Separation of Targeted Algal

2015-4-17  Hydrocyclone appears to be a viable alterative to existing algal dewatering efforts that can bring down algal harvesting costs Hydrocylones are expected to be employed at large-scale outdoor algal facilities to replace settling, dissolved air flotation, and centrifuge unit operations Hydrocyclone gains are realized

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Separation of CHO cells using hydrocyclones SpringerLink

2007-11-14  High cell separation efficiencies, varying from 97.9% to 99.9%, were obtained for 5 out of 6 hydrocyclone configurations. The geometry 3010 (D u = 3.0 mm and D o = 1.0 mm) presented a flow ratio of 100%, diverting the total feed flow to the underflow, indicating that this configuration is inappropriate to perform any kind of cell separation. . Loss of viability was within the range of 2.9–9 ...

More

Separation of CHO cells using hydrocyclones. - Abstract ...

2007-11-14  The cell number balances (comparison of the amount of cells leaving the hydrocyclone through the underflow and overflow streams with the amount of cells fed to the equipment) closed satisfactorily, with an average deviation of ±9.9%, which is approximately the same deviation associated to the cell counting technique in Neubauer chambers used ...

More

separation: Application to primary harvesting of ...

2017-6-2  Brief comparison has been made between the conventional techniques for primary harvesting of microalgae based on the concentration factor and power consumption in the table S3. Technique Concentration factor Power consumption (kWh/m3) Continuous Ref Macro-hydrocyclone 4 0.3 Yes (1) Decanter bowl centrifuge 11 8 No (2) Vacuum filter 2-18.5 - Yes ...

More

Use of Hydrocyclones for Mammalian Cell Retention ...

2006-8-1  A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply). Cultures of BHK and HeLa ...

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Hydrocyclone for mAb production in a perfusion single

2020-10-20  For the first time, a hydrocyclone specially designed for mammalian cell separation is reported to enable perfusion processes at cell densities in the range of 20 to 50 million/mL for 20 to 25 days. Also for the first time, a HC is connected to a single-use bioreactor.

More

(PDF) Separation of CHO cells using hydrocyclones ...

Separation of CHO cells using hydrocyclones. Rodrigo Pinto. Related Papers. Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) ... Characterizing hydrocyclone performance for grit removal from wastewater treatment activated sludge plants.

More

Microalgae harvesting techniques: A review - ScienceDirect

2018-7-1  The harvesting method intensely depends on the physiognomies of the micro algae chosen, density and size of the microalgal cell, specifications of the final product and on allowability for reuse of the culture medium (Uduman et al., 2010, Amaro et al., 2011, Rawat et al., 2011).

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Very High Density of CHO Cells in Perfusion by ATF or

density was applied. The cells were maintained by cell bleeds at density 0.9–1.3 3 108 cells/mL in growing state and at high viability for more than 2 weeks. Finally, with the pres-ent settings, maximal cell densities of 2.14 3 108 cells/mL, achieved for the first time in a

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